Discrepancy between structural characteristics and ligand specificity of an African catfish gonadotropin receptor
Bogerd, J.; Andersson, E.; Blomenröhr, M.; Tensen, C.P.; Van der Putten, H.H.A.G.M.; Vischer, H.; Granneman, J.C.M.; Jansen-Dommerholt, C.; Goos, H.J.Th.; Schulz, R.W. (2000). Discrepancy between structural characteristics and ligand specificity of an African catfish gonadotropin receptor, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 167-169
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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| Authors | | Top |
- Bogerd, J.
- Andersson, E.
- Blomenröhr, M.
- Tensen, C.P.
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- Van der Putten, H.H.A.G.M.
- Vischer, H.
- Granneman, J.C.M.
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- Jansen-Dommerholt, C.
- Goos, H.J.Th.
- Schulz, R.W.
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| Abstract |
A 2.0 kb putative testicular gonadotropin receptor cDNA was cloned from African catfish. Alignment of the deduced amino acid sequence with other glycoprotein hormone receptors and analysis of the catfish gene indicated that the cloned receptor belonged to the FSH-R subfamily. The cDNA encoded a functional receptor since its transient expression in HEK-T 293 cells resulted in ligand-dependent cAMP production (order of potency: catfish LH [cfLH] > human recombinant FSH [hrFSH]; human choriogonadotropin [hCG], human recombinant LH [hrLH] and human TSH [hTSH] were inactive). However, hCG (as well as cfLH, hrFSH, pregnant mare serum gonadotropin [PMSG], but not hTSH) stimulates testicular androgen secretion in vitro, indicating that a second (LH-R-like) type of gonadotropin receptor is expressed in African catfish. We conclude that we have cloned a cDNA encoding a functional catfish testicular gonadotropin receptor. Although the cloned receptor displays the structural characteristics of a FSH-R, it can be activated by catfish LH. |
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