Fish pituitary glycoprotein hormone receptors: cloning and characterization of two different gonadotropin receptors from gonads and two thyrotropin-like receptors from thyroid of amago salmon (Oncorhynchus rhodurus)
Oba, Y.; Hirai, T.; Yoshiura, Y.; Nagahama, Y. (2000). Fish pituitary glycoprotein hormone receptors: cloning and characterization of two different gonadotropin receptors from gonads and two thyrotropin-like receptors from thyroid of amago salmon (Oncorhynchus rhodurus), in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 164-166
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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| Authors | | Top |
- Oba, Y.
- Hirai, T.
- Yoshiura, Y.
- Nagahama, Y.
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| Abstract |
Two different types of gonadotropin receptor cDNAs (sGTH-RI and sGTH-RII), and two different types of thyrotropin receptor-like cDNAs (sTSH-RA and sTSH-RB) were cloned from amago salmon ovary and thyroid. Phylogenetic analysis grouped sGTH-RI and -RII with mammalian FSH and LH receptors, and sTSH-RA and -RB with mammalian TSH receptors. Transient transfection studies revealed that the corresponding ligand (sGTH-RI, GTH I; sGTH-RII, GTH II; sTSH-RA and -RB, bovine TSH) was most potent in stimulation of cAMP synthesis. Northern blot analysis was performed to measure sGTH-RI and -RII mRNA during ovarian development. The sGTH-RI gene was expressed during vitellogenesis, and the sGTH-RII gene was expressed strongly in late-vitellogenic and full-grown stages. After ovulation, sGTH-RI mRNA levels were high. |
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