Continuous genomic surveillance monitored the in vivo evolutionary trajectories of Vibrio parahaemolyticus and identified a new virulent genotype
Fu, S.; Yang, Q.; Wang, Q.; Pang, B.; Lan, R.; Wei, D.; Qu, B.; Liu, Y. (2021). Continuous genomic surveillance monitored the in vivo evolutionary trajectories of Vibrio parahaemolyticus and identified a new virulent genotype. mSystems 6(1): e01254-20. https://dx.doi.org/10.1128/msystems.01254-20
In: mSystems. American Society for Microbiology: Washington, DC. e-ISSN 2379-5077, more
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| Keywords |
Vibrio parahaemolyticus (Fujino, Okuno, Nakada, Aoyama, Fukai, Mukai & Ueho, 1951) Sakazaki, Iwanami & Fukumi, 1963 [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Fu, S.
- Yang, Q., more
- Wang, Q.
- Pang, B.
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- Lan, R.
- Wei, D.
- Qu, B.
- Liu, Y.
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| Abstract |
Our ability to predict evolutionary trajectories of pathogens is one of the promising leverages to fight against the pandemic disease, yet few studies have addressed this question in situ, due to the difficulty in monitoring the milestone evolutionary events for a given pathogen and in understanding the evolutionary strategies. In this study, we monitored the real-time evolution of Vibrio parahaemolyticus in response to successive antibiotic treatment in three shrimp farms in North China from 2011 to 2018 by whole-genome sequencing. Results showed that the stepwise emergence of resistance was associated with the antibiotic usage. Genomic analysis of resistant isolates showed that the acquisition of the resistant mobile genetic elements flanked by an insertion sequence (ISVal1) closely mirrored the antibiotics used in shrimp farms since 2014. Next, we also identified 50 insertion sites of ISVal1 in the chromosome, which facilitated the formation of pathogenicity islands (PAIs) and fitness islands in the following years. Further, horizontal transfers of a virulent trh-nik-ure genomic island (GI) and two GIs improving the fitness have been observed in two farms since 2016. In this case study, we proposed that the insertion sequence triggered four major evolutionary events during the outbreaks of shrimp disease in three farms, including horizontal transfer of transposon (HTT) (stage 1), the formation of resistance islands (stage 2) and the PAIs (stage 3), and horizontal transfer of the PAIs (stage 4). This study presented the first in vivo evolutionary trajectories for a given bacterial pathogen, which helps us to understand the emergence mechanisms of new genotypes. |
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