The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host
Krause, S.; Gfrerer, S.; von Kügelgen, A.; Reuse, C.; Dombrowski, N.; Villanueva, L.; Bunk, B.; Spröer, C.; Neu, T.R.; Kuhlicke, U.; Schmidt-Hohagen, K.; Hiller, K.; Bharat, T.A.M.; Rachel, R.; Spang, A.; Gescher, J. (2022). The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host. Nature Comm. 13: 1735. https://dx.doi.org/10.1038/s41467-022-29263-y
Additional data:
In: Nature Communications. Nature Publishing Group: London. ISSN 2041-1723; e-ISSN 2041-1723, more
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| Authors | | Top |
- Krause, S.
- Gfrerer, S.
- von Kügelgen, A.
- Reuse, C.
- Dombrowski, N., more
- Villanueva, L., more
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- Bunk, B.
- Spröer, C.
- Neu, T.R.
- Kuhlicke, U.
- Schmidt-Hohagen, K.
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- Hiller, K.
- Bharat, T.A.M.
- Rachel, R.
- Spang, A., more
- Gescher, J.
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| Abstract |
Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterised microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of theMicrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses.
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