IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (1): add | show Print this page
one publication added to basket [257856]
Rapid enumeration of Escherichia coli in marine bathing waters: potential interference of nontarget bacteria
Baudart, J.; Servais, P.; De Paoli, H.; Henry, A.; Lebaron, P. (2009). Rapid enumeration of Escherichia coli in marine bathing waters: potential interference of nontarget bacteria. J. Appl. Microbiol. 107(6): 2054-2062. dx.doi.org/10.1111/j.1365-2672.2009.04392.x
In: Journal of Applied Microbiology. Blackwell Science: Oxford. ISSN 1364-5072; e-ISSN 1365-2672, more
Peer reviewed article  
Available in  Authors 
Keywords
    Escherichia coli Castellani & Chalmers, 1919 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]
    Marine/Coastal
Author keywords
    bathing waters; Escherichia coli; glucuronidase activity; real-timemonitoring; Vibrio
Authors  Top 
  • Baudart, J.
  • Servais, P., more
  • De Paoli, H.
  • Henry, A.
  • Lebaron, P.
Abstract
    Aims: To compare the Escherichia coli quantification given by the ‘Coliplage®’ assay, based on the direct measurement of the ß-d-glucuronidase (GLUase) activity and the reference Most Probable Number (MPN) method from seawater sites and investigate the possible interference of non-E. coli strains in the GLUase activity measurement. Methods and Results: Comparison performed from 69 French coastal bathing sites (1401 samples) showed nonconcordance between both methods, only for 8% of samples. Non-E. coli 4-methylumbelliferyl-ß-d-glucuronide (MUG+) were isolated from nonconcordant samples. Phylogenetic analysis showed that Gammaproteobacteria were dominants and mainly represented by Vibrio species, which displayed GLUase activities on the same order of magnitude and sometimes much higher as E. coli reference strains. Conclusions: The‘Coliplage®’ assay is a rapid method for the quantification of E. coli showed few discordances with the standard MPN method. Some Vibrio species could interfere on the direct GLUase activity measurement of E. coli. Significance and Impact of the Study: Data present the first qualitative investigation on disagreement between Coliplage® and the MPN results. If the interference of Vibrio species is confirmed in situ, appropriate treatments should be developed to remove the interfering signal.
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors