IMIS

The dataset consists of two parts: 1. The raw data cleaned and filtered according to requirements for NCBI submission. The raw data are .fastq files and represent the output from the Illumina NovaSeq 6000 sequencing. For each sample (16, 55, 60, 21 are the ID number of samples in the file name) there are F1 and F2 since the sequencing was 100 bp paired-end. 2. The final heater tissue transcriptome and relative statistics. The raw reads were treated as follows: sequencing raw reads were submitted to a conservative pipeline of quality control (Fastqc, Trimmomatic, Rcorrector and Centrifuge). In the end of pre-processing stage, only raw reads with phred score ≥ 28 were kept for downstream analysis. The de-novo transcriptome assembly was built with the Trinity assembler (default parameters) and decontaminated using previously stablished methods (Machado et al., 2019). The transcriptome assessment was done with Transrate and BUSCO (benchmarking universal single-copy orthologs) tools.