Rapid screening of marine bacterial symbionts using MALDI-TOF MS
Vidal, L.M.R.; Venas, T.M.; Goncalves, A.R.P.; Mattsson, H.K.; Silva, R.V.P.; Nobrega, M.S.; Azevedo, G.P.R.; Garcia, G.D.; Tschoeke, D.A.; Vieira, V.V.; Thompson, F.L.; Thompson, C.C. (2020). Rapid screening of marine bacterial symbionts using MALDI-TOF MS. Arch. Microbiol. 202(8): 2329-2336. https://dx.doi.org/10.1007/s00203-020-01917-9
In: Archives of Microbiology. Springer: Heidelberg; Berlin. ISSN 0302-8933; e-ISSN 1432-072X, more
|  |
| Keywords |
Vibrio Pacini, 1854 [WoRMS]
Marine/Coastal |
| Author keywords |
MALDI-TOF; Screening tool; Marine bacterial symbionts; Vibrio; pyrHgene |
| Authors | | Top |
- Vidal, L.M.R.
- Venas, T.M.
- Goncalves, A.R.P.
- Mattsson, H.K.
|
- Silva, R.V.P.
- Nobrega, M.S.
- Azevedo, G.P.R.
- Garcia, G.D.
|
- Tschoeke, D.A.
- Vieira, V.V.
- Thompson, F.L.
- Thompson, C.C.
|
| Abstract |
Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups’ identification. |
|
