Development of a compact experimental system for ecotoxicological experiments on Acropora spp.
Takeuchi, I.; Takayama, K. (2023). Development of a compact experimental system for ecotoxicological experiments on Acropora spp., in: Takeuchi, I. et al. Coral reefs of Eastern Asia under anthropogenic impacts. Coral Reefs of the World, 17: pp. 139-151. https://dx.doi.org/10.1007/978-3-031-27560-9_8
In: Takeuchi, I.; Yamashiro, H. (Ed.) (2023). Coral reefs of Eastern Asia under anthropogenic impacts. Coral Reefs of the World, 17. Springer Nature: Switzerland. ISBN 978-3-031-27559-3; e-ISBN 978-3-031-27560-9. VII, 180 pp. https://dx.doi.org/10.1007/978-3-031-27560-9, more
In: Coral Reefs of the World. Springer: Dordrecht. ISSN 2213-719X, more
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| Keywords |
Acropora Oken, 1815 [WoRMS]
Marine/Coastal |
| Author keywords |
Acropora spp.; artificial seawater; colour evaluation; coral bleaching; experimental system; filtered tap water |
| Authors | | Top |
- Takeuchi, I.
- Takayama, K.
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| Abstract |
The present chapter describes a compact experimental system for ecotoxicological experiments that use small clones of Acropora spp. Our system comprises widely accessible inexpensive equipment and devices. Tap water provided by the local government waterworks agency and coastal seawater in Japan is potentially contaminated with various herbicides and pesticides. Thus, we prepared artificial seawater using tap water filtered more than twice through water purifier. To avoid contamination by anionic surfactants, Petri dishes and other experimental devices were washed using acetone, hexane, and HCl. A rapid, straightforward method for attaching small clones of Acropora spp. to polycarbonate bolts using an ultraviolet light-curable oligomer-based adhesive is described. This method enables the prompt and precise measurements of the maximum effective quantum yield (ΔF/Fm′) using a pulse amplitude modulated (PAM) fluorometer. The evaluation of coral colour based on RGB values (R, red; G, green; B, blue; each ranging from 0 to 255) of digital images acquired manually by a digital camera is described. This experimental system enables us to conduct various experiments, including recording of the bleaching process in corals exposed to high temperature and herbicides and gene expression analysis of Acropora exposed to herbicides, in a laboratory located far from coral reefs in a subtropical-tropical zone. Our system will advance the analysis of corals exposed to various anthropogenic factors. |
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