Sposensor: A whole-bacterial biosensor that uses immobilized Bacillus subtilis spores and a one-step incubation/detection process
Fantino, J.-R.; Barras, F.; Denizot, F. (2009). Sposensor: A whole-bacterial biosensor that uses immobilized Bacillus subtilis spores and a one-step incubation/detection process. J. Mol. Microbiol. Biotechnol. 17(2): 90-95. https://dx.doi.org/10.1159/000206634
In: Journal of Molecular Microbiology and Biotechnology. Karger: Basel. ISSN 1464-1801; e-ISSN 1660-2412, more
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| Keyword |
Bacillus subtilis Cohen, 1872 [WoRMS]
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| Authors | | Top |
- Fantino, J.-R.
- Barras, F.
- Denizot, F.
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| Abstract |
A generic whole-cell bacterial sensor called sposensor was developed with immobilized spores from engineered Bacillus subtilis. Sposensor contains two different types of spores: reporting spores that contain a reporter gene fused to a promoter responding to a compound to be detected, and control spores use to monitor cell germination and viability. A one-step incubation/detection process was developed to meet the constraints of on-site analysis. Spores were directly incubated with culture medium containing the compound to be detected. beta-Galactosidase was chosen as a reporter protein in both cases and its activity followed by a colorimetric assay. Results showed that sposensor was efficient in detecting two different compounds, a metal (Zn(2+)) and a peptidic antibiotic (bacitracin). Owing to the stability and robustness of spores, sposensor is a very efficient and easy tool to manipulate for analyzing the presence of toxic compounds in natural settings. |
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