A blind comparison of simple bench-top bioassays and human tumour cell cytotoxicities as antitumor prescreens
Anderson, J.E.; Goetz, C.M.; McLaughlin, J.L.; Suffness, M. (1991). A blind comparison of simple bench-top bioassays and human tumour cell cytotoxicities as antitumor prescreens. Phytochemical Analysis 2(3): 107-111. https://dx.doi.org/10.1002/pca.2800020303
In: Phytochemical Analysis. Wiley-Blackwell: Hoboken. ISSN 0958-0344; e-ISSN 1099-1565, more
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| Keywords |
Agrobacterium tumefaciens; Artemia salina (Linnaeus, 1758) [WoRMS]; Lemna minor
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| Authors | | Top |
- Anderson, J.E.
- Goetz, C.M.
- McLaughlin, J.L.
- Suffness, M.
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| Abstract |
Simple bench-top bioassays involving brine shrimp lethality, Lemna frond proliferation, and the inhibition of crown gall tumours on potato discs, as well as the human tumour cell lines A-549 lung carcinoma, MCF-7 breast carcinoma and HT-29 colon adenocarcinoma, were compared for their accuracy to detect known in vivo (P-388) active antitumour agents supplied by the National Cancer Institute. The potato disc assay was the best and showed excellent correlation to in vivo activity (p=0.008). The brine shrimp assay (p = 0.033) proved to be superior or equally as accurate as the in vitro human solid tumour cell lines (p = 0.033-0.334). The Lemna assay (p = 0.708) showed the poorest correlation. The brine shrimp and potato disc assays are suggested as convenient in-house prescreens to existing cytotoxicity or antitumour assays. |
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