Gene editing in the ascidian Phallusia mammillata and tail nerve cord formation
McDougall, A.; Hebras, C.; Gomes, I.; Dumollard, R. (2021). Gene editing in the ascidian Phallusia mammillata and tail nerve cord formation, in: Carroll, D.J. et al. Developmental biology of the sea urchin and other marine invertebrates: Methods and protocols. Methods in Molecular Biology, 2219: pp. 217-230. https://dx.doi.org/10.1007/978-1-0716-0974-3_13
In: Carroll, D.J.; Stricker, S.A. (Ed.) (2021). Developmental biology of the sea urchin and other marine invertebrates: Methods and protocols. Second Edition. Methods in Molecular Biology, 2219. Humana Press: New York. ISBN 978-1-0716-0976-7. XIII, 309 pp. https://dx.doi.org/10.1007/978-1-0716-0974-3, more
In: Methods in Molecular Biology. Humana Press. ISSN 1064-3745, more
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| Keywords |
Phallusia mammillata (Cuvier, 1815) [WoRMS]
Marine/Coastal |
| Author keywords |
Ascidian; Gene editing; CRISPR-Cas9; Microinjection; Nerve cord |
| Authors | | Top |
- McDougall, A.
- Hebras, C.
- Gomes, I.
- Dumollard, R.
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| Abstract |
Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata. |
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