Exploring a blue-light-sensing transcription factor to double the peak productivity of oil in Nannochloropsis oceanica
Zhang, P.; Xin, Y.; He, Y.; Tang, X.; Shen, C.; Wang, Q.; Lv, N.; Li, Y.; Hu, Q.; Xu, J. (2022). Exploring a blue-light-sensing transcription factor to double the peak productivity of oil in Nannochloropsis oceanica. Nature Comm. 13: 1664. https://dx.doi.org/10.1038/s41467-022-29337-x
In: Nature Communications. Nature Publishing Group: London. ISSN 2041-1723; e-ISSN 2041-1723, more
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| Keywords |
Nannochloropsis oceanica Suda & Miyashita, 2002 [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Zhang, P.
- Xin, Y.
- He, Y.
- Tang, X.
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| Abstract |
Oleaginous microalgae can produce triacylglycerol (TAG) under stress, yet the underlying mechanism remains largely unknown. Here, we show that, in Nannochloropsis oceanica, a bZIP-family regulator NobZIP77 represses the transcription of a type-2 diacylgycerol acyltransferase encoding gene NoDGAT2B under nitrogen-repletion (N+), while nitrogen-depletion (N−) relieves such inhibition and activates NoDGAT2B expression and synthesis of TAG preferably from C16:1. Intriguingly, NobZIP77 is a sensor of blue light (BL), which reduces binding of NobZIP77 to the NoDGAT2B-promoter, unleashes NoDGAT2B and elevates TAG under N−. Under N+ and white light, NobZIP77 knockout fully preserves cell growth rate and nearly triples TAG productivity. Moreover, exposing the NobZIP77-knockout line to BL under N− can double the peak productivity of TAG. These results underscore the potential of coupling light quality to oil synthesis in feedstock or bioprocess development. |
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