Bacterial toxicity testing: modification and evaluation of the luminescent bacteria test and the respiration inhibition test
Strotmann, U.; Flores, D.P.; Konrad, O.; Gendig, C. (2020). Bacterial toxicity testing: modification and evaluation of the luminescent bacteria test and the respiration inhibition test. Processes 8(11): 1349. https://hdl.handle.net/10.3390/pr8111349
In: Processes. MDPI AG: Basel. ISSN 2227-9717; e-ISSN 2227-9717, more
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| Author keywords |
bacterial toxicity; chlorinated phenols; Cr(VI) and Zn(II) cations; luminescent bacteria; respiration inhibition; activated sludge; long-term toxicity |
| Authors | | Top |
- Strotmann, U.
- Flores, D.P., more
- Konrad, O.
- Gendig, C.
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| Abstract |
The activated sludge respiration inhibition test and the luminescent bacteria test with Vibrio fischeri are important bacterial test systems for evaluation of the toxicity of chemical compounds. These test systems were further optimized to result in better handling, reliability and sensitivity. Concerning the Vibrio fischeri test, media components such as yeast extract and bivalent cation concentrations like Ca2+ and Mg2+ were optimized. The cultivation, storage conditions and reactivation process of the stored bacteria were also improved, which enabled simpler handling and led to good reproducibility. Additionally, the respiration inhibition test with a prolonged incubation time was further analyzed using different chlorinated phenols as reference compounds. It could be stated that a longer incubation period significantly improved the sensitivity of the test system. |
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