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β-hydroxyacyl-CoA-dehydrogenase (HADH) differentiates unfrozen from frozen-thawed crawfish (Procambarus clarkii) and trout (Salmo gairdneri) meat
Hoz, L.; Yustes, C.; Cámara, J.M.; Ramos, M.A.; García de Fernando, G.D. (1992). β-hydroxyacyl-CoA-dehydrogenase (HADH) differentiates unfrozen from frozen-thawed crawfish (Procambarus clarkii) and trout (Salmo gairdneri) meat. Int. J. Food Sci. Technol. 27(2): 133-136. https://dx.doi.org/10.1111/j.1365-2621.1992.tb01188.x
In: International Journal of Food Science and Technology. Blackwell Scientific: Oxford. ISSN 0950-5423; e-ISSN 1365-2621, more
Peer reviewed article  

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Keywords
    Procambarus clarkii (Girard, 1852) [WoRMS]; Salmo gairdnerii Richardson, 1836 [WoRMS]
Author keywords
    Enzyme test, fresh fish detection

Authors  Top 
  • Hoz, L.
  • Yustes, C.
  • Cámara, J.M.
  • Ramos, M.A.
  • García de Fernando, G.D.

Abstract
    An enzymatic method for differentiation of unfrozen from thawed crawfish (Procambarus clarkii ) and trout (Salmo gairdneri ) has been developed. The method is based on the release of the β‐hydroxyacyl‐CoA‐dehydrogenase (HADH) during freezing. The HADH activity mean values were 7.9 and 78.9 for unfrozen and thawed trout, respectively. In the case of crawfish, these values were 12 and 70 respectively. Statistical analyses showed significant differences (P 0.001) in both species. Trout could be distinguished with a high confidence level, but for crawfish this level was slightly lower.

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