Morphological and molecular study of Contracaecum larvae with a new record of Contracaecum septentrionale in Al-Sanaf marsh southern Thi-Qar Province, Iraq
Mohammad, Z.A.-A.; Hbaiel, M.K. (2019). Morphological and molecular study of Contracaecum larvae with a new record of Contracaecum septentrionale in Al-Sanaf marsh southern Thi-Qar Province, Iraq. Indian Journal of Public Health Research & Development 10(10): 1271-1276. https://dx.doi.org/10.5958/0976-5506.2019.03007.9
In: Indian Journal of Public Health Research & Development. Institute of Medico-Legal Publications: New Delhi. ISSN 0976-0245; e-ISSN 0976-5506, more
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| Keywords |
Developmental stages > Larvae
Iraq
Contracaecum septentrionale Kreis, 1955 [WoRMS]
Iraq [Marine Regions]
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| Author keywords |
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| Authors | | Top |
- Mohammad, Z.A.-A.
- Hbaiel, M.K.
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| Abstract |
Contracaecum nematodes are the most significantgenus of the Anisakidae because of their wide range of host species involved in their life cycles, large number of their species and their health impacts. The current study aims to present the morphological andmolecular characters offourth stage larvae of Contracaecum that isolated from N. nycticorax proventriculus as free larvae in al-Sanaf marsh, southern Iraq. Larvae of Contracaecum spp. collected from proventriculus of N. nycticorax aquatic birds werestudied morphologically and their genomic DNA isolated, the ITS-1 regions of rDNA amplified by PCR technique. Sequences alignments carried out to screen nucleotide variation in ITS-1 among samples. The alignment of the sequences between Contracaecum larvae in current study with the previously reported reference gene sequences in the GenBank showed two distinct species. The first Contracaecum septentrionale recorded for the first time in Iraq and submitted to GenBank with the accession numbers MK424799.1, the second Contracaecum microcephalumsubmitted to GenBank with the accession numbers MK424795. The analysis of the ITS-1 data for Contracaecum larvae supported the distinction between species of Contracaecum larvae. |
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