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Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages
Heres, P.; Rodriguez-Riveiro, R.; Troncoso, J.; Paredes, E. (2019). Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages. Cryobiology 86: 40-46. https://dx.doi.org/10.1016/j.cryobiol.2019.01.001
In: Cryobiology. Academic Press: San Diego. ISSN 0011-2240; e-ISSN 1090-2392, more
Peer reviewed article  
Available in  Authors 
Keywords
    ASSEMBLEPlus Joint Research Activity 2
    Scientific Publication
    Mytilus galloprovincialis Lamarck, 1819 [WoRMS]
Author keywords
    Mussel, Cryopreservation, Cryoprotecting agents, Marine invertebrates, Toxicity
Authors  Top 
  • Heres, P.
  • Rodriguez-Riveiro, R.
  • Troncoso, J.
  • Paredes, E.
Abstract
    Global aquaculture production of blue mussel has increased over last years. This work reaffirms the great potential of cryopreservation technique on mussel industry and overcome economic barriers a cause of a traditional and rudimentary management and continue growing. The aim of this work is to set some preliminary basis attending to toxicity of cryoprotecting agents (CPAs) on different development stages of Mytilus galloprovincialis as a start point to develop a stable cryopreservation protocol. Toxicity tests were carried out by using common CPAs (dimethyl-sulfoxide (Me2SO), glycerol, (GLY), propylene glycol (PG) and ethylene glycol (EG)) in a range from 0.5 to 3 M on fertilized egg, trochophore larva, and D-larva of Mytilus galloprovincialis. Results evidenced more resistance of older development stages to toxicity. Of all CPAs tested, toxicity testing highlights PG or EG as suitable CPAs for cryopreservation of early development stages; whereas D-larva was unaffected by any of the CPAs tested. Preliminary cryopreservation trials were developed to obtain information into cell cryoprotection. Further research should be focused on membrane permeability and other parameters, such as the balance between toxicity and cryoprotective effect of CPAs.
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