Sampling of riverine or marine bacterial communities in remote locations: from field to publication
Lehmann, K. (2016). Sampling of riverine or marine bacterial communities in remote locations: from field to publication, in: Bourlat, S.J. (Ed.) Marine genomics: methods and protocols. Methods in Molecular Biology, 1452: pp. 1-11. https://dx.doi.org/10.1007/978-1-4939-3774-5_1
In: Bourlat, S.J. (Ed.) (2016). Marine genomics: methods and protocols. Methods in Molecular Biology, 1452. Humana Press/Springer Science+Business Media, Inc: New York. ISBN 978-1-4939-3772-1. 253 pp. https://dx.doi.org/10.1007/978-1-4939-3774-5, more
In: Methods in Molecular Biology. Humana Press. ISSN 1064-3745, more
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| Author keywords |
Biodiversity; Riverine microbial communities; Community analysis; Illumina; MiSeq |
| Abstract |
This protocol describes how to sample and preserve microbial water column samples from rivers that can be used for 16S or 18S metabarcoding studies or shotgun sequencing. It further describes how to extract the DNA for sequencing and how to prepare raw Illumina MiSeq amplicon data and analyze it in the R environment. |
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