Transcriptome sequencing and annotation of the polychaete Hermodice carunculata (Annelida, Amphinomidae)
Mehr, S.; Verdes, A.; DeSalle, R.; Sparks, J.; Pieribone, V.; Gruber, D.F. (2015). Transcriptome sequencing and annotation of the polychaete Hermodice carunculata (Annelida, Amphinomidae). BMC Genom. 16(1): 445. https://dx.doi.org/10.1186/s12864-015-1565-6
In: BMC Genomics. BioMed Central: London. e-ISSN 1471-2164, more
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| Keywords |
Hermodice carunculata (Pallas, 1766) [WoRMS]; Polychaeta [WoRMS]
Marine/Coastal |
| Author keywords |
Next-generation sequencing, Hermodice carunculata, Polychaete, Molecular phylogenetics, de novo assembly, Functional annotation |
| Authors | | Top |
- Mehr, S.
- Verdes, A.
- DeSalle, R.
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- Sparks, J.
- Pieribone, V.
- Gruber, D.F.
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| Abstract |
The amphinomid polychaete Hermodice carunculata is a cosmopolitan and ecologically important omnivore in coral reef ecosystems, preying on a diverse suite of reef organisms and potentially acting as a vector for coral disease. While amphinomids are a key group for determining the root of the Annelida, their phylogenetic position has been difficult to resolve, and their publically available genomic data was scarce. We performed deep transcriptome sequencing (Illumina HiSeq) and profiling on Hermodice carunculata collected in the Western Atlantic Ocean. We focused this study on 58,454 predicted Open Reading Frames (ORFs) of genes longer than 200 amino acids for our homology search, and Gene Ontology (GO) terms and InterPro IDs were assigned to 32,500 of these ORFs. We used this de novo assembled transcriptome to recover major signaling pathways and housekeeping genes. We also identify a suite of H. carunculata genes related to reproduction and immune response. We provide a comprehensive catalogue of annotated genes for Hermodice carunculata and expand the knowledge of reproduction and immune response genes in annelids, in general. Overall, this study vastly expands the available genomic data for H. carunculata, of which previously consisted of only 279 nucleotide sequences in NCBI. This underscores the utility of Illumina sequencing for de novo transcriptome assembly in non-model organisms as a cost-effective and efficient tool for gene discovery and downstream applications, such as phylogenetic analysis and gene expression profiling. |
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