Hydrolysis of Nothogenia erinacea xylan by xylanases from families 10 and 11
Nerinckx, W.; Broberg, A.; Duus, J.Ø; Ntarima, P.; Parolis, L.A.S.; Parolis, H.; Claeyssens, M. (2004). Hydrolysis of Nothogenia erinacea xylan by xylanases from families 10 and 11. Carbohydrate Research 339(6): 1047-1060. https://dx.doi.org/10.1016/j.carres.2004.02.017
In: Carbohydrate Research. Elsevier SCI Ltd: Oxford. ISSN 0008-6215; e-ISSN 1873-426X, more
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| Keyword |
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| Author keywords |
xylanases; seaweed; xylooligosaccharides; hydrolysis; rhodymenan |
| Authors | | Top |
- Nerinckx, W.
- Broberg, A.
- Duus, J.Ø
- Ntarima, P.
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- Parolis, L.A.S.
- Parolis, H.
- Claeyssens, M.
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| Abstract |
The structures of several enzymatic hydrolysis products of Nothogenia erinacea seaweed xylan, a linear homopolymer with mixed β-(1 → 3)/β-(1 → 4) linkages, were analysed by physicochemical and biochemical techniques. With the glycoside hydrolase family 10 β-(1 → 4)-xylanase from Cryptococcus adeliae, hydrolysis proceeds to a final mixture of products containing a mixed linkage-type triose as a major compound, whereas with the family 11 xylanase from Thermomyces lanuginosus this is a mixed linkage tetraose. The Cryptococcus xylanase is shown to be capable of also catalysing the hydrolysis of β-(1 → 3) linkages, that is this of a mixed type tetraose intermediary formed, in accordance with the broader substrate specificity of family 10 enzymes. From a partial degradation experiment with the T. lanuginosus xylanase, a series of higher mixed oligosaccharides were isolated and identified. The observed oligosaccharide intermediates and splicing pattern indicate an irregular β-(1 → 3)/β-(1 → 4) linkage distribution within the linear d-xylose polymer. Similar results were obtained with rhodymenan, the seaweed xylan from Palmares palmata. |
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