Extraction of ATP from aufwuchs communities
Clark, J.R.; Messenger, D.I.; Dickson, K.L.; Cairns, Jr., J. (1978). Extraction of ATP from aufwuchs communities. Limnol. Oceanogr. 23(5): 1055-1059
In: Limnology and Oceanography. American Society of Limnology and Oceanography: Waco, Tex., etc. ISSN 0024-3590; e-ISSN 1939-5590, more
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| Keywords |
Aquatic communities
Chemical compounds > Phosphorus compounds > Phosphates > Nucleotides > ATP
Population characteristics > Biomass
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| Authors | | Top |
- Clark, J.R.
- Messenger, D.I.
- Dickson, K.L.
- Cairns, Jr., J.
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| Abstract |
Acetone extracts prepared by an acid-ether elution procedure for ATP analysis by the firefly luminescence reaction yielded estimates comparable to those of the boiling tris buffer method and an acetone distillation technique, from algal and fungal cultures and colonized glass slides. The acetone acid-ether technique was more effective than the other methods in extracting ATP from aufwuchs attached to rock substrates. The technique allows estimates of ATP and chlorophyll to be made from a single sample. |
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