Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution
In: Journal of Fish Biology. Fisheries Society of the British Isles: London,New York,. ISSN 0022-1112; e-ISSN 1095-8649, more
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| Keywords |
Biological stress
Enzymatic activity
Enzymes
Fish culture
Handling > Fish handling
Pollution > Water pollution
Stress
Transportation
Oncorhynchus mykiss (Walbaum, 1792) [WoRMS]
Fresh water |
| Abstract |
Blood lysozyme activity was determined in rainbow trout (Salmo gairdneri) that were stressed by handling and transport, or suffered from acute ammonia poisoning. To optimize the lysoplate assay, the pH dependence of the lysozyme activity was studied. For serum and kidney lysozyme, the pH optimum was 5 multiplied by 5 to 6 multiplied by 0. Ventilation frequency and plasma glucose concentration of the fish were used as stress indicators. Less severely stressful situations, such as 30 min of handling, brought about either a stimulation or a suppression of the lysozyme activity. Strong stressors, such as transport lasting 2 h or acute water pollution, reduced the lysozyme level significantly. The decrease of lysozyme activity due to handling and transport persisted for 24 h, but the activity returned to normal within 2 weeks following confrontation with the stressor. |
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