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Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak
Citation
Rahlff, J.; Esser, S.P.; Heinrichs, M.E.; Giebel, HA.; Probst, A.J.; (2023): Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak. PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.947348
Availability:
 This dataset is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.Description
Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. more
Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022. Scope Themes: Biology > Pleuston > Neuston Keywords: Marine/Coastal · Fresh water · Air-sea · ANE, Skagerrak · Bacterial abundance · Sea foam · Sea-surface microlayer · Virus-like particles · Skagerrak Geographical coverage Skagerrak [Marine Regions] Temporal coverage
3 February 2020 - 26 February 2020 Parameters
Virus-like particles Methodology
Eukaryotes, phototrophic, small Methodology Prokaryotes Methodology Salinity Methodology Water temperature Methodology Wind speed Methodology Virus-like particles: Flow cytometer, BD Biosciences, BD Accuri C6 Eukaryotes, phototrophic, small: Flow cytometer, BD Biosciences, BD Accuri C6 Prokaryotes: Flow cytometer, BD Biosciences, BD Accuri C6 Salinity: Thermosalinometer, portable, WTW Multiline 3420 Water temperature: Thermosalinometer, portable, WTW Multiline 3420 Wind speed: Anemometer, AN-10, Voltcraft Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, more
Funding Horizon Europe
Grant agreement ID 730984
Publication
Based on this dataset
Rahlff, J. et al. (2023). Marine viruses disperse bidirectionally along the natural water cycle. Nature Comm. 14(1): 6354. https://dx.doi.org/10.1038/s41467-023-42125-5, more
Rahlff, J. et al. (2022). Heads in the clouds: marine viruses disperse bidirectionally along the natural water cycle. bioRxiv 2022: 1-35. https://dx.doi.org/10.1101/2022.06.21.497027 , more
Dataset status: Completed
Data type: Metadata
Data origin: Research: field survey
Metadatarecord created: 2023-05-11
Information last updated: 2024-02-05
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