Effects of chicken-II gonadotropin releasing hormone and a GnRH antagonist on spawning behavior of the female goldfish, Carassius auratus
Volkoff, H.; Peter, R.E. (2000). Effects of chicken-II gonadotropin releasing hormone and a GnRH antagonist on spawning behavior of the female goldfish, Carassius auratus, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 129-131
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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| Abstract |
The central effects of a native form of gonadotropin-releasing hormone (GnRH), chicken GnRH-II (cGnRH-II), and a GnRH antagonist, [Ac-delta 3-Pro, 4FD-Phe, D-Trp]-mGnRH (analog E) on the spawning behavior of sexually recrudescent female goldfish were investigated. Spawning behavior was induced by intramuscular injection of females with prostaglandin F sub(2 alpha ) and placing them with mature males. Behavioral responses were quantified by recording the numbers of spawning acts performed by each pair of fish during two hours following brain intracerebroventricular (ICV) injection of GnRH peptides or saline as control. Each pair of fish was pre-tested to determine their level of spawning behavior, for comparison to spawning behavior following treatment. ICV injections of analog E at 5ng/g caused a significant decrease in the number of spawning acts performed by females, suggesting a role of endogenous GnRH in modulating spawning behavior. Injections of cGnRH-II at 0.5ng/g significantly enhanced female spawning behavior. Analog E suppressed the actions of exogenous cGnRH-II on spawning behavior, as the cGnRH-II-induced increase in number of spawning acts was inhibited by concomitant treatment with analog E. These results indicate that GnRH plays a role in the control of female reproductive behavior in goldfish. |
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