Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus
Pesce, A.; Nardini, M.; Dewilde, S.; Ascenzi, P.; Riggs, A.F.; Yamauchi, K.; Geuens, E.; Moens, L.; Bolgnesi, M. (2001). Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus. Acta Crystallographica Section D-Structural Biology 57: 1897-1899. dx.doi.org/10.1107/S0907444901015815
In: Acta Crystallographica Section D-Structural Biology. International Union of Crystallography: Oxford. ISSN 2059-7983, more
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| Keyword |
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| Author keywords |
neural haemoglobin; mini haemoglobin; Fe anomalous signals |
| Authors | | Top |
- Pesce, A.
- Nardini, M.
- Dewilde, S., more
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- Ascenzi, P.
- Riggs, A.F.
- Yamauchi, K.
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- Geuens, E.
- Moens, L., more
- Bolgnesi, M.
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| Abstract |
The nemertean worm Cerebratulus lacteus neural tissue haemoglobin (109 amino acids, the shortest known haemoglobin) has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant native data set has been collected at the Cu K wavelength to 2.05 Å resolution. The crystals belong to the orthorhombic P212121 space group, with unit-cell parameters a = 42.5, b = 43.1, c = 60.2 Å and one molecule per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe atom, thus paving the way for MAD/SAD structure determination. |
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