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Three-dimensional reconstruction of the tissue-specific multielemental distribution within Ceriodaphnia dubiavia multimodal registration using laser ablation ICP-mass spectrometry and X-ray spectroscopic techniques
Van Malderen, S.J.M.; Laforce, B.; Van Acker, T.; Nys, C.; De Rijcke, M.; de Rycke, R.; De Bruyne, M.; Boone, M.N.; De Schamphelaere, K.; Borovinskaya, O.; De Samber, B.; Vincze, L.; Vanhaecke, F. (2017). Three-dimensional reconstruction of the tissue-specific multielemental distribution within Ceriodaphnia dubiavia multimodal registration using laser ablation ICP-mass spectrometry and X-ray spectroscopic techniques. Anal. Chem. 89(7): 4161-4168. https://dx.doi.org/10.1021/acs.analchem.7b00111
In: Analytical chemistry. American Chemical Society: Washington. ISSN 0003-2700; e-ISSN 1520-6882
Peer reviewed article  
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Auteurs  Top 
  • Van Malderen, S.J.M.
  • Laforce, B.
  • Van Acker, T.
  • Nys, C.
  • De Rijcke, M.
  • de Rycke, R.
  • De Bruyne, M.
  • Boone, M.N.
  • De Schamphelaere, K.
  • Borovinskaya, O.
  • De Samber, B.
  • Vincze, L.
  • Vanhaecke, F.
Abstract
    In this work, the three-dimensional elemental, distribution profile within the freshwater crustacean Ceriodaphnia dubia was constructed at a spatial resolution down to S mu m via a data, fusion approach employing state-of-the-art laser ablation inductively coupled plasma-time-of-flight mass spectrometry (LAICP-TOFMS) and laboratory-based absorption microcomputed tomography (mu-CT). C. dubia was exposed to elevated Cu, Ni, and Zn concentrations, chemically fixed, dehydrated, stained, and embedded, prior to mu-CT analysis. Subsequently, the sample was cut into 5 pm thin sections that were subjected to LA-ICPTOFMS imaging. Multimodal image registration was performed to spatially align the 2D LA-ICP-TOFMS images relative to the Corresponding slices of the 3D mu-CT reconstruction. Mass channels corresponding to the isotopes of a single element were merged to improve the signal-to-noise ratios within the elemental images. In order to aid the visual interpretation of the data, LA-ICP-TOEMS data wete projected onto the mu-CT voxels representing tissue. Additionally, the image resolution and elemental sensitivity were compared to those obtained with synchrotron radiation based 3D confocal mu-X-ray fluorescence imaging upon a chemically fixed and air-dried C. dubia specimen.
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