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Characterisation of vitellogenin in Arctic char
Berg, A.H.; Westerlund, L.; Norlin, M.; Olsson, P.-E. (2000). Characterisation of vitellogenin in Arctic char, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 365
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Berg, A.H.
  • Westerlund, L.
  • Norlin, M.
  • Olsson, P.-E.
Abstract
    The present study was aimed at characterising VTG from Arctic char (Salvelinus alpinus). VTG was purified from plasma of 17- beta -estradiol treated juvenile Arctic char and used to obtain polyclonal antibodies. A cDNA library constructed from estrogen treated Arctic char liver was screened for VTG and several clones with high homology to rainbow trout (Oncorhynchus mykiss) VTG were isolated. The sequences suggest that the isolated clones code for at least two different VTG transcripts. The obtained VTG cDNA and the antibodies are being used to study the effect of substances with suspected estrogenic effect on vitellogenesis in Arctic char.
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