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Monoamine metabolism in the hypothalamus of the catfish, Pimelodus blochii, during gonadal development
Rodriguez, Ch.; Fontaine, M.; Cardillo, E.; Marcano, D. (2000). Monoamine metabolism in the hypothalamus of the catfish, Pimelodus blochii, during gonadal development, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 63
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer

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  • Rodriguez, Ch.
  • Fontaine, M.
  • Cardillo, E.
  • Marcano, D.

Abstract
    The Catfish, Pimelodus blochii, is a seasonal breeder widely distributed in rivers of the Venezuelan plains. Under natural conditions, it have an annual gonadal cycle closely related to seasonal changes during the dry and rainy season. The purpose of this study was to evaluate the changes in the metabolism of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) in the hypothalamus of P. blochii. Amine levels have been correlated with gonadal stages (based on visual inspection). Adult male and female P. blochii (Teleostei, Siluriformes) were collected on a regular basis from February to July 1998 from Cano Igueez, Portuguesa State in Venezuelan plains. Animals were sacrificed by decapitation, brains removed and the Hypothalamus (H), dissected. The whole hypothalamus was homogenised (at a final concentration 100 mg/ml) in ice-cold 0.2 M perchloric acid and 0.1 mM EDTA

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