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Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin
Vannerum, K.; Huysman, M.J.J.; De Rycke, R.; Vuylsteke, M.; Leliaert, F.; Pollier, J.; Lütz-Meindl, U.; Gillard, J.; De Veylder, L.; Goossens, A.; Inzé, D.; Vyverman, W. (2011). Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin. BMC Plant Biol. 11(128). dx.doi.org/10.1186/1471-2229-11-128
In: BMC Plant Biology. BioMed Central: London. ISSN 1471-2229; e-ISSN 1471-2229
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Growth > Cell growth
    Morphogenesis
    Micrasterias C.Agardh ex Ralfs, 1848 [WoRMS]
    Marien/Kust
Author keywords
    Expansin
Auteurs  Top 
  • Vannerum, K.
  • Huysman, M.J.J.
  • De Rycke, R.
  • Vuylsteke, M., meer
  • Leliaert, F.
  • Pollier, J.
  • Lütz-Meindl, U.
  • Gillard, J.
  • De Veylder, L.
  • Goossens, A.
  • Inzé, D.
  • Vyverman, W., meer
Abstract
    BackgroundStreptophyte green algae share several characteristics of cell growth and cell wall formation with their relatives, the embryophytic land plants. The multilobed cell wall of Micrasterias denticulata that rebuilds symmetrically after cell division and consists of pectin and cellulose, makes this unicellular streptophyte alga an interesting model system to study the molecular controls on cell shape and cell wall formation in green plants.ResultsGenome-wide transcript expression profiling of synchronously growing cells identified 107 genes of which the expression correlated with the growth phase. Four transcripts showed high similarity to expansins that had not been examined previously in green algae. Phylogenetic analysis suggests that these genes are most closely related to the plant EXPANSIN A family, although their domain organization is very divergent. A GFP-tagged version of the expansin-resembling protein MdEXP2 localized to the cell wall and in Golgi-derived vesicles. Overexpression phenotypes ranged from lobe elongation to loss of growth polarity and planarity. These results indicate that MdEXP2 can alter the cell wall structure and, thus, might have a function related to that of land plant expansins during cell morphogenesis.ConclusionsOur study demonstrates the potential of M. denticulata as a unicellular model system, in which cell growth mechanisms have been discovered similar to those in land plants. Additionally, evidence is provided that the evolutionary origins of many cell wall components and regulatory genes in embryophytes precede the colonization of land.
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