Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate
Malissard, M.; Duez, C.; Guinand, M.; Vacheron, M.-J.; Michel, G.; Marty, N.; Joris, B.; Thamm, I.; Ghuysen, J.-M. (1993). Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate. FEMS Microbiol. Lett. 110(1): 101-106. https://hdl.handle.net/10.1111/j.1574-6968.1993.tb06302.x
The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originated from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870–1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385–8391).
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