NARMS source details

Cárdenas, P.; Moore, J.A. (2019). First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens. Marine Biodiversity. 49 (1): 163-174.
283612
10.1007/s12526-017-0775-3 [view]
Cárdenas, P.; Moore, J.A.
2019
First records of <i>Geodia</i> demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens
Marine Biodiversity
49 (1): 163-174
Publication
We report the first records of the sponge genus Geodia (Demospongiae, Tetractinellida, Geodiidae) from the New England Seamounts and Muir Seamount, at lower bathyal depths. Nine specimens collected between 2000 and 2005 belong to two boreal species (Geodia macandrewii and Geodia barretti) and a temperate species (Geodia megastrella). These records extend the distributions of these deep-sea amphi-Atlantic species to the west. Most of these specimens were originally fixed in formalin, which substantially degraded the DNA. We nonetheless managed to sequence two cytochrome c oxidase subunit I (COI) mini-barcodes: the universal mini-barcode at the 5' end of the Folmer barcode (130 bp) and a newly proposed mini-barcode at the 3' end of the Folmer barcode (296 bp). These mini-barcodes unambiguously confirmed our identifications. As an additional test, we also successfully sequenced these two mini-barcodes from the holotype of G. barretti, collected in 1855. We conclude by advocating the use of mini-barcodes on formalin-fixed or old specimens with degraded DNA.
America, North
Boreal western Atlantic
Systematics, Taxonomy
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Date
action
by
2017-08-26 13:57:55Z
created
2017-12-16 08:17:49Z
changed
2019-06-07 15:18:10Z
changed

Geodia barretti Bowerbank, 1858 (additional source)
Geodia macandrewii Bowerbank, 1858 (additional source)
Geodia megastrella Carter, 1876 (additional source)