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Data on long term survival of cryopreserved mussel larvae (Mytilus galloprovinciallis)
Citatie
Rodriguez-Riveiro, R.; Heres, P.; Troncoso, J.; Paredes, E.; Estación de Ciencias Mariñas de Toralla (Uvigo-ECIMAT): Spain; (2021): Data on long term survival of cryopreserved mussel larvae (Mytilus galloprovinciallis). https://marineinfo.org/id/dataset/7901

Gearchiveerde data
Beschikbaarheid: Creative Commons License Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.

Beschrijving
This dataset is part of the package of data linked to the cryopreservation protocols developed by JRA2 of ASSEMBLE Plus. The data (excel spreadsheet) are the measurements made during the crypopreservation tests. The aim of this study was to develop a successful cryopreservation protocol for Mytilus galloprovincialis larvae and study long-term effects by assessing survival and development post cryobanking. meer

Due to the economic importance of the mussel Mytilus galloprovincialis in the Spanish aquaculture, there is a growing interest in developing alternative methods to ensure the supply of good-quality biological material and to minimize the uncertainty and variability that might be a source of economic risk for this sector. We evaluated the effect of previously studied cryoprotecting agent, Ethylene glycol (EG) for cryopreservation of trochophore larvae and D-veliger larvae 48h and 72h old, following an existing preliminary protocol for M. galloprovincialis mussel trochophores. The protocol selected for cryopreservation consisted on holding at 4°C for 2min, then cooling at 1°C/min to −12°C, holding for 5min, then cooling at −35°C with a rate of 1°C/min, then plunging into liquid nitrogen for storage. Thawing took place by immersion in 35°C water bath. With this protocol the initial percentage of short-term developed a D-larvae (48h incubation post-thaw) was 48.9+/-7.6% with 10% EG + 0.2M Trehalose (TRE). This initial test only studied the trochophore larvae until they reached the D-stage (48h old); in the present experiment experiments, we replicated this study but also carried out a long-term larval rearing with cryopreserved trochophore larvae in comparison to fresh larvae. This experiment allowed a comparative post-freezing analysis of both larval development and survival. Larval settlement was also characterized after 13days. The data revealed that until settling, the survival of the cryopreserved larvae was slightly lower than the 2.8% reported in the larval rearing for Greenshell mussel. Over time, there was an initial difference in size of cryopreserved larvae when compared to controls, but from day 17 onwards the size between both types of larvae began to stabilize. Attending to settlement, we obtained a 64% of success of cryopreserved larvae respect to the control.

Scope
Thema's:
Biologie > Invertebraten
Kernwoorden:
Marien/Kust, ASSEMBLEPlus Joint Research Activity 2, Cryopreservation, Data, Larval rearing, Trochophore larvae, Mytilus galloprovincialis Lamarck, 1819

Spreiding in de tijd
16 November 2017 - 7 December 2017

Taxonomic coverage
Mytilus galloprovincialis Lamarck, 1819 [WoRMS]

Parameter
Count of larvae

Bijdrage door
University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla (ECIMAT), meerdata creator

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, meer

Publicatie
Gebaseerd op deze dataset
Rodriguez-Riveiro, R. et al. (2019). Long term survival of cryopreserved mussel larvae (Mytilus galloprovinciallis). Aquaculture 512: 734326. https://dx.doi.org/10.1016/j.aquaculture.2019.734326, meer

Dataset status: Afgelopen
Data type: Data
Data oorsprong: Onderzoek: labo-experiment
Metadatarecord aangemaakt: 2021-10-18
Informatie laatst gewijzigd: 2022-10-18
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