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Sampling site specific biomarker responses in Mediterranean mussels from the Adriatic Sea
Citeerbaar als data publicatie
Mitric, M.; Ramšak A.; University of Montenegro, Institute of Marine Biology: Montenegro; National Institute of Biology, Marine Biology Station Piran: Slovenia; (2020): Sampling site specific biomarker responses in Mediterranean mussels from the Adriatic Sea. Marine Data Archive. https://doi.org/10.14284/439

Beschikbaarheid: Creative Commons License Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.

Beschrijving
The obtained data sets (condition index, metallothionein concentrations, the activity of acetylcholinesterase (EC 3.1.1.7), micronuclei frequency) representing biomarker responses (metallothioneins, acetylcholinesterase and micronuclei) measured during early spring and late summer in Mytilus galloprovincialis, sampled from two ecosystems between 2009 and 2012, both part of the Adriatic Sea (Gulf of Trieste and Boka Kotorska Bay). Environmental parameters (seawater temperature, salinity and dissolved oxygen) were measured at the time of mussels sampling being important parameters to interpret biomarker responses. meer

Sampling sites were selected according to previous knowledge on pollution pressures in the Gulf of Trieste (Bay of Koper (ST1), Bay of Strunjan (ST2), Bay of Piran (ST3)) and the Boka Kotorska Bay (Bay of Kotor (ST4) and Bay of Herceg Novi (ST5)). Reference sites were ST2 and ST4. Sampling time was from 2009 to 2012 in early spring (in March) and late summer (in September). The size of the collected mussels ranges from 40 to 60 mm. Metallothionein (MT) concentrations were measured in composite samples made of 50 mussels and expressed as micrograms of metallothionein per gram of tissue wet weight (µg/g wet weight). Acetylcholinesterase activity was measured in hepatopancreas or gills of individual mussels and was expressed in nmol of degraded acetylcholine per milligrams of proteins per minute (nmol/min/mg protein). Micronucleus (MN) were investigated in gills of 10 mussels pere sampling site and expressed as the frequency in promiles. Condition index (CI) was measured in twenty mussels from each sampling site and expressed in mg/cm3. Abiotic parameters of seawater (temperature, salinity and dissolved oxygen content) were measured by multiparameter probe MSS 90 (Sea & Sun Technology) at the time of mussel sampling at the depth of 2 m at all sampling sites in the Gulf of Trieste and with Handheld Multi-Parameter Instrument, Multi 350i (WTW, Xylem Brand) in the Boka Kotorska, respectively.


Dataset is acknowledged to scientific collaboration between Slovenia and Montenegro in years 2010-2011, agreement BI-ME/10-11-7 and the financial support from the Slovenian Research Agency; research core funding No. P1-0237 Coastal Sea Research.


Scope
Thema's:
Biologie > Benthos, Watersamenstelling
Kernwoorden:
Marien/Kust, Abiotic parameters, Acetylcholinesterase, Biomarkers, Biomonitoring, Condition index, Metallothioneins, Micronuclei, MED, Adriatic Sea, MED, Adriatic, Boka Kotorska Bay, MED, Adriatic, Gulf of Trieste, Mytilus galloprovincialis Lamarck, 1819

Geografische spreiding
MED, Adriatic Sea [Marine Regions]
MED, Adriatic, Boka Kotorska Bay [Marine Regions]
MED, Adriatic, Gulf of Trieste [Marine Regions]

Spreiding in de tijd
September 2009 - September 2012
Tweemaal per jaar

Taxonomic coverage
Mytilus galloprovincialis Lamarck, 1819 [WoRMS]

Parameters
Acetylcholinesterase Methode , Condition index Methode , Metallothioneins Methode , Micronucleus Methode , Opgeloste zuurstof Methode , Saliniteit Methode , Temperatuur Methode
Acetylcholinesterase: Tsangaris, C. et al. (2016). https://doi.org/10.1007/s11356-015-5410-x
Condition index: Lobel, P. B. et al. (1991). Improved protocol for collecting mussel watch specimens taking into account sex, size, condition, shell shape, and chronological age. Archives of Environmental Contamination and Toxicology, 21(3), 409-414
Metallothioneins: Viarengo, A. et al. (1997). https://doi.org/10.1016/S0141-1136(96)00103-1
Micronucleus: Bolognesi, C.; Fenech, M. (2012). https://doi.org/10.1038/nprot.2012.043
Opgeloste zuurstof: MSS 90 multiparameter probe (Sea & Sun Technology) in the Gulf of Trieste (ST1, ST3, ST3)
Opgeloste zuurstof: Multi 350i handheld multi-parameter instrument (WTW, Xylem Brand) in the Boka Kotorska Bay (ST4, ST5)
Saliniteit: Multi 350i handheld multi-parameter instrument (WTW, Xylem Brand) in the Boka Kotorska Bay (ST4, ST5)
Saliniteit: MSS 90 multiparameter probe (Sea & Sun Technology) in the Gulf of Trieste (ST1, ST3, ST3)
Temperatuur: Multi 350i handheld multi-parameter instrument (WTW, Xylem Brand) in the Boka Kotorska Bay (ST4, ST5)
Temperatuur: MSS 90 multiparameter probe (Sea & Sun Technology) in the Gulf of Trieste (ST1, ST3, ST3)

Bijdrage door
National Institute of Biology; Marine Biological Station Piran (MBS), meerdata creator
University of Montenegro; Institute of Marine Biology, meerdata creator

Publicatie
Gebaseerd op deze dataset
Mitric, M.; Ramšak, A. (2021). Sampling site specific biomarker responses in Mediterranean mussels from the Adriatic Sea. Bull. Environ. Contam. Toxicol. 106(2): 310-317. https://dx.doi.org/10.1007/s00128-020-03083-6, meer

Dataset status: Afgelopen
Data type: Data
Data oorsprong: Onderzoek: labo-experiment
Metadatarecord aangemaakt: 2020-12-06
Informatie laatst gewijzigd: 2020-12-07
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