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Genotoxicological parameters in Mytilus galloprovincialis
Citatie
Kolarević S.; Kračun-Kolarević M.; Ramšak A.; Bajt O.; Institute for Biological Research ''Sinisa Stanković'' National Institute of Republic of Serbia: Serbia; Marine Biology Station Piran, National Institute of Biology: Slovenia; (2020): Genotoxicological parameters in Mytilus galloprovincialis. Marine Data Archive.

Beschikbaarheid: Vrij beschikbaar na embargo periode
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Beschrijving
Genotoxicological parameters measured in M. galloprovincialis upon exposure to microplastic particles coupled with fluoranthene. meer

The major objective of the project is studying of the effects of microplastics and adsorbed pollutants on Mediterranean mussel (Mytilus galloprovincialis) as sentinel species. More precisely, the extent of effects is measured under ecological relevant scenario for the Northern Adriatic, simulated in the controlled experimental conditions. The focus is placed on DNA damage as an endpoint of specific interest in indicated scenario. Acclimated mussels with intact byssus threads were divided in groups per 30 specimens and exposed as following: 1. Control aquarium: 100 μL of DMSO and 20 μL of Tween 80 was added. 2. Polysttyrene : 100 mg of polystyrene particles was dissolved in 5 mL of seawater from aquaria spiked with 20 μL of Tween 80. Additionally, 100 μL of DMSO was added to aquarium. 3. For fluoranthene 100 μL of stock solutions (10 mg/mL) prepared in DMSO was added to corresponding aquaria. Additionaly 20 μL of Tween 80 was added to each aquarium. 4. Particles with adsorbed fluoranthene were resuspended in 4 mL of seawater taken from aquaria. To each tube, 20 μL of Tween-80 was added to prevent clumping of the particles. Additionally 100 μL of DMSO was added. Specimens were exposed for 96 h where checkpoints for analyses were set to be after 24, 48, 72 and 96 h of treatment. Haemolymph was used for genotoxicological assays. Comet assay was at each checkpoint while micronucleus test was performed only after 96h of treatment.

Scope
Kernwoorden:
Marien/Kust, ASSEMBLEPlus Transnational Access, Cornet assay, Data, Genotoxicological assay, Micronucleus, Microplastics, MED, Adriatic, North, Mytilus galloprovincialis Lamarck, 1819

Geografische spreiding
MED, Adriatic, North [Marine Regions]

Spreiding in de tijd
19 Juli 2019 - 19 Juli 2020

Taxonomic coverage
Mytilus galloprovincialis Lamarck, 1819 [WoRMS]

Parameters
DNA damage Methode
DNA damage: Cornet Assay
DNA damage: Micronucleus test

Bijdrage door
National Institute of Biology; Marine Biological Station Piran (MBS), meerdata creator

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, meer

Dataset status: Gestart
Data type: Data
Data oorsprong: Onderzoek: labo-experiment
Metadatarecord aangemaakt: 2020-07-20
Informatie laatst gewijzigd: 2020-12-07
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