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Illuminating adaptive divergence through hybrid zones in Littorina saxatilis
Citatie
Raffini, F.; Butlin, R.; Department of Animal and Plant Sciences. University of Sheffield: United Kingdom; (2019): Illuminating adaptive divergence through hybrid zones in Littorina saxatilis. http://www.assembleplus.eu/information-system?module=dataset&dasid=6372

Beschikbaarheid: Vrij beschikbaar na embargo periode
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Beschrijving
Dataset describing position, habitat and date of collection, snail phenotype-genotype data, and positions and habitats at movement observation and recapture seks and developmental stage data. meer

64 snails were collected in the field (Centinela, Baiona, Spain; coordinates: N 42° 4' 38.06", W 8° 53' 47.47") in the wave, crab and hybrid zone respectively (total sample size: 192). Prior to collection, the habitat and position of each individual was recorded with a survey instrument (total station) to <10cm resolution. Specimens were transported to the lab where they were marked with individual waterproof labels and non-toxic nail polish along the margin of the aperture (to track growth); shell thickness was measured with a gauge; shells photographed for morphology; and DNA collected from mucus (non-destructive DNA sampling protocol using TFA cards). Individuals were then released in the field: each collection group (crab/hybrid/wave) was randomly split in three subgroups and released in the hybrid, crab and wave habitats, respectively, recording the precise release position and habitat characteristics. One day later, the positions and habitats of marked snails was recorded for movement. This round of collection-mark-release was repeated four times (one release included only 64 snails from the hybrid zone due to not permissive weather), for a final number of 640 released snails. At the end of the visit (a total of 14 days, surviving snails plus three individuals released in a previous round of sampling in June were recaptured, with positions and habitats recorded, phenotyped (growth, weight) and dissected for sex determination and tissue collection for additional DNA analysis.

Scope
Thema's:
Biologie > Benthos
Kernwoorden:
Marien/Kust, Adaptation, divergent selection, genomic architecture, Habitat choice, Hybrid zones, Parallel evolution, Reproductive isolation, Speciation, Spain, Vigo, Littorina saxatilis (Olivi, 1792)

Geografische spreiding
Spain, Vigo

Spreiding in de tijd
13 Juni 2019 - 19 Juni 2019
21 September 2019 - 6 Oktober 2019

Taxonomic coverage
Littorina saxatilis (Olivi, 1792) [WoRMS]

Parameters
Genotype, Geslacht, Gewicht, Groei, Habitat, Lichaamsafmetingen, Ontwikkelingsstadia in populatie

Bijdrage door
University of Sheffield; School of Biological Sciences; Department of Animal and Plant Sciences (APS), meerdata creator

Gerelateerde datasets
Andere relatie:
Insights from hybrid zones: testing for habitat choice, divergent selection and genomic architecture in Littorina saxatilis adaptive divergence, meer

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, meer

Dataset status: Afgelopen
Data type: Data
Data oorsprong: Onderzoek: veldexperiment
Metadatarecord aangemaakt: 2019-12-03
Informatie laatst gewijzigd: 2020-04-16
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