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Insights from hybrid zones: testing for habitat choice, divergent selection and genomic architecture in Littorina saxatilis adaptive divergence
Citatie
Raffini, F.; Butlin, R.; Department of Animal and Plant Sciences. University of Sheffield: United Kingdom; (2019): Insights from hybrid zones: testing for habitat choice, divergent selection and genomic architecture in Littorina saxatilis adaptive divergence. http://www.assembleplus.eu/information-system?module=dataset&dasid=6371

Beschikbaarheid: Vrij beschikbaar na embargo periode
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Beschrijving
Dataset describing position, habitat and date of collection, snail phenotype-genotype data, and positions and habitats at movement observation and recapture seks and developmental stage data. meer

48 snails were collected in the field (Centinela, Baiona, Spain; coordinates: N 42° 4' 38.06", W 8° 53' 47.47") in the wave, crab and hybrid zone respectively (total sample size: 144). Prior to collection, the habitat and position of each individual was recorded with a survey instrument (total station) to <10cm resolution. Specimens were transported to the lab where they were marked with individual waterproof labels and non-toxic nail polish along the margin of the aperture (to track growth); shell thickness was measured with a gauge; shells photographed for morphology; and DNA collected from mucus (non-destructive DNA sampling protocol using TFA cards). Individuals were then released in the field: each collection group (crab/hybrid/wave) was randomly split in three subgroups and released in the hybrid, crab and wave habitats, respectively, recording the precise release position and habitat characteristics. One day later, the positions and habitats of marked snails was recorded for movement. This round of collection-mark-release was repeated four times (one release included only 48 snails from the hybrid zone due to not permissive weather), for a final number of 480 released snails. One month later, in June, surviving snails were recaptured, with positions and habitats recorded, phenotyped (growth, weight) and dissected for sex determination and tissue collection for additional DNA analysis.

Scope
Thema's:
Biologie > Benthos
Kernwoorden:
Marien/Kust, Adaptation, divergent selection, genomic architecture, Habitat choice, Hybrid zones, Parallel evolution, Reproductive isolation, Speciation, Spain, Vigo, Littorina saxatilis (Olivi, 1792)

Geografische spreiding
Spain, Vigo

Spreiding in de tijd
23 Maart 2019 - 11 April 2019
1 Juni 2019 - 10 Juni 2019

Taxonomic coverage
Littorina saxatilis (Olivi, 1792) [WoRMS]

Parameters
Genotype, Geslacht, Gewicht, Groei, Habitat, Lichaamsafmetingen, Ontwikkelingsstadia in populatie

Bijdrage door
University of Sheffield; School of Biological Sciences; Department of Animal and Plant Sciences (APS), meerdata creator

Gerelateerde datasets
Andere relatie:
Illuminating adaptive divergence through hybrid zones in Littorina saxatilis, meer

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, meer

Dataset status: Afgelopen
Data type: Data
Data oorsprong: Onderzoek: veldexperiment
Metadatarecord aangemaakt: 2019-12-03
Informatie laatst gewijzigd: 2020-04-16
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