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Androgenesis in sterlet (Acipenser ruthenus) using fresh and cryopreserved sperm
Urbányi, B.; Horváth, Á.; Bercsényi, M.; Horváth, L. (2000). Androgenesis in sterlet (Acipenser ruthenus) using fresh and cryopreserved sperm, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 440
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Urbányi, B.
  • Horváth, Á.
  • Bercsényi, M.
  • Horváth, L.
Abstract
    Successful diploid androgenesis was accomplished on sterlet (Acipenser ruthenus L.). The eggs of wild coloured females were irradiated using gamma radiation. Sperm of albino coloured males was used either fresh or cryopreserved for the fertilisation of irradiated eggs. A heat shock was applied to the eggs to restore the diploid state of embryos. Parameters of irradiation dosage, heat shock duration, temperature and the time between fertilisation and shocking were optimised. The percentage of hatched larvae varied between 3.1 and 5.3 % in these experiments. All hatched larvae inherited the albino coloration of the male parent
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