Model for a novel membrane envelope in a filamentous hyperthermophilic virus
Kasson, P.; DiMaio, F.; Yu, X.; Lucas-Staat, S.; Krupovic, M.; Schouten, S.; Prangishvili, D.; Egelman, E.H. (2017). Model for a novel membrane envelope in a filamentous hyperthermophilic virus. eLIFE 6: e26268. https://dx.doi.org/10.7554/eLife.26268.001
In: eLIFE. eLife Sciences Publications: Cambridge. e-ISSN 2050-084X, more
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| Authors | | Top |
- Kasson, P.
- DiMaio, F.
- Yu, X.
- Lucas-Staat, S.
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- Krupovic, M.
- Schouten, S., more
- Prangishvili, D.
- Egelman, E.H.
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| Abstract |
Biological membranes create compartments, and are usually formed by lipid bilayers.However, in hyperthermophilic archaea that live optimally at temperatures above 80°C themembranes are monolayers which resemble fused bilayers. Many double-stranded DNA viruseswhich parasitize such hosts, including the filamentous virus AFV1 of Acidianus hospitalis, areenveloped with a lipid-containing membrane. Using cryo-EM, we show that the membrane in AFV1is a ~2 nm-thick monolayer, approximately half the expected membrane thickness, formed by hostmembrane-derived lipids which adopt a U-shaped ‘horseshoe’ conformation. We hypothesize thatthis unusual viral envelope structure results from the extreme curvature of the viral capsid, as‘horseshoe’ lipid conformations favor such curvature and host membrane lipids that permithorseshoe conformations are selectively recruited into the viral envelope. The unusual envelopefound in AFV1 also has many implications for biotechnology, since this membrane can survive themost aggressive conditions involving extremes of temperature and pH. |
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