A method for determining exoproteolytic activity in natural waters
In: Limnology and Oceanography. American Society of Limnology and Oceanography: Waco, Tex., etc. ISSN 0024-3590; e-ISSN 1939-5590, meer
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| Auteurs | | Top |
- Somville, M.
- Billen, G., meer
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| Abstract |
A very sensitive method allowing determination of exoproteolytic activity in natural water is based on the use of aminoacyl-ß-naphthylamide, which gives rise to a fluorescent product upon hydrolysis of its peptidelike bond by filtered or unfiltered water samples. Specificity of the method to exoproteases is demonstrated by a strong competitive inhibition effect of added proteins.A good correlation is found between exoproteolytic activity and the rate of amino acid utilization by microheterotrophs in various aquatic environments. |
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