IMIS | Vlaams Instituut voor de Zee
 

Vlaams Instituut voor de Zee

Platform voor marien onderzoek

IMIS

Publicaties | Instituten | Personen | Datasets | Projecten | Kaarten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Isolation and characterisation of 14 novel microsatellite markers through Next Generation Sequencing for the commercial Atlantic seabob shrimp Xiphopenaeus kroyeri
Kerkhove, T.; Hellemans, B.; De Troch, M.; De Backer, A.; Volckaert, F.A.M. (2019). Isolation and characterisation of 14 novel microsatellite markers through Next Generation Sequencing for the commercial Atlantic seabob shrimp Xiphopenaeus kroyeri. Molecular biology reports 46(6): 6565-6569. https://hdl.handle.net/10.1007/s11033-019-05026-9
In: Molecular biology reports. ISSN 0301-4851; e-ISSN 1573-4978, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
Author keywords
    Population structure; Shotgun sequencing; DNA microsatellites; Sustainable fisheries; Western Atlantic

Auteurs  Top 
  • De Backer, A., meer
  • Volckaert, F.A.M., meer

Abstract
    Assessing population genetic structure is a crucial step to support fisheries and conservation management. DNA microsatellite molecular markers are a widely used tool in population genotyping. In the present study, we characterised and developed 14 novel polymorphic microsatellite markers for a decapod crustacean, the Atlantic seabob shrimp Xiphopenaeus kroyeri (Heller, 1862), through rapid and cost-effective Illumina shotgun sequencing and a Galaxy-based bioinformatic pipeline. We genotyped 60 individuals from 2 populations with the newly developed microsatellites, resulting in the detection of 3 to 29 alleles per locus. Four loci deviated from Hardy–Weinberg equilibrium. Cross-amplification in a cryptic congeneric species was successful for eight loci (57%). The microsatellite loci developed in this study will be highly relevant for genetic and evolutionary studies of X. kroyeri, and for the stock management of this commercially exploited species.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs