IMIS | Vlaams Instituut voor de Zee
 

Vlaams Instituut voor de Zee

Platform voor marien onderzoek

IMIS

Publicaties | Instituten | Personen | Datasets | Projecten | Kaarten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sorfjord, Norway)
Catarino, A.I.; Cabral, H.; Peeters, K.; Pernet, P.; Punjabi, U.; Dubois, P. (2008). Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sorfjord, Norway). Environ. Toxicol. Chem. 27(7): 1553-1560. https://dx.doi.org/10.1897/07-402.1
In: Environmental Toxicology and Chemistry. Setac Press: New York. ISSN 0730-7268; e-ISSN 1552-8618, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
    Asterias rubens Linnaeus, 1758 [WoRMS]; Echinus acutus Lamarck, 1816 [WoRMS]
    Marien
Author keywords
    Asterias rubens; Echinus acutus; metals; RNA/DNA ratio; sperm motility

Auteurs  Top 
  • Catarino, A.I., meer
  • Cabral, H.
  • Peeters, K.
  • Pernet, P., meer
  • Punjabi, U.
  • Dubois, P., meer

Abstract
    The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs