IMIS | Vlaams Instituut voor de Zee

Vlaams Instituut voor de Zee

Platform voor marien onderzoek


Publicaties | Instituten | Personen | Datasets | Projecten | Kaarten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

CALUX measurements: statistical inferences for the dose–response curve
Elskens, M.; Baston, D.S.; Stumpf, C.; Haedrich, J.; Keupers, I.; Croes, K.; Denison, M.S.; Baeyens, W.; Goeyens, L. (2011). CALUX measurements: statistical inferences for the dose–response curve. Talanta 85(4): 1966-1973.
In: Talanta. Elsevier: Amsterdam. ISSN 0039-9140; e-ISSN 1873-3573, meer
Peer reviewed article  

Beschikbaar in  Auteurs 
    VLIZ: Open Repository 279669 [ OMA ]

Author keywords
    Bioassay CALUX; Statistical inference; Dose–response curve; Box–Cox transformation

Auteurs  Top 
  • Elskens, M., meer
  • Baston, D.S., meer
  • Stumpf, C.
  • Haedrich, J.
  • Keupers, I., meer
  • Croes, K., meer
  • Denison, M.S.
  • Baeyens, W., meer
  • Goeyens, L., meer

    Chemical Activated LUciferase gene eXpression [CALUX] is a reporter gene mammalian cell bioassay used for detection and semi-quantitative analyses of dioxin-like compounds. CALUX dose–response curves for 2,3,7,8-tetrachlorodibenzo-p-dioxin [TCDD] are typically smooth and sigmoidal when the dose is portrayed on a logarithmic scale. Non-linear regression models are used to calibrate the CALUX response versus TCDD standards and to convert the sample response into Bioanalytical EQuivalents (BEQs). Several complications may arise in terms of statistical inference, specifically and most important is the uncertainty assessment of the predicted BEQ. This paper presents the use of linear calibration functions based on Box–Cox transformations to overcome the issue of uncertainty assessment. Main issues being addressed are (i) confidence and prediction intervals for the CALUX response, (ii) confidence and prediction intervals for the predicted BEQ-value, and (iii) detection/estimation capabilities for the sigmoid and linearized models. Statistical comparisons between different calculation methods involving inverse prediction, effective concentration ratios (ECR20–50–80) and slope ratio were achieved with example datasets in order to provide guidance for optimizing BEQ determinations and expand assay performance with the recombinant mouse hepatoma CALUX cell line H1L6.1c3.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs