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Biological and chemical approaches for the detection and identification of illegal estrogens in water based solutions
Noppe, H.; Arijs, K.; De Wasch, K.; Van Cruchten, S.; Poelmans, S.; Courtheyn, D.; Cobbaert, E.; Gillis, W.; Vanthemsche, P.; De Brabander, H.F.; Janssen, C.R.; Van Hoof, N. (2006). Biological and chemical approaches for the detection and identification of illegal estrogens in water based solutions. Vet. Res. Comm. 30(6): 577-585. https://dx.doi.org/10.1007/s11259-006-3326-0
In: Veterinary Research Communications. Springer: Dordrecht; Tokyo; Oxford; New York; London; Boston; Amsterdam. ISSN 0165-7380; e-ISSN 1573-7446, meer
Is gerelateerd aan:
Noppe, H.; Arijs, K.; De Wasch, K.; Van Cruchten, S.; Poelmans, S.; Courtheyn, D.; Cobbaert, E.; Gillis, W.; Vanthemsche, P.; De Brabander, H.F.; Janssen, C.R.; Van Hoof, N. (2006). Biological and chemical approaches for the detection and identification of illegal estrogens in water based solutions, in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 59-72, meer
Peer reviewed article  

Beschikbaar in  Auteurs 
    VLIZ: Open Repository 280370 [ OMA ]

Trefwoorden
    Detection
    Estrogens
    Identification
Author keywords
    animal trials; anatomy; GC-MSn; histology; LC-MSn; YES

Project Top | Auteurs 
  • Endocrine disruption in the Scheldt Estuary: distribution, exposure and effects, meer

Auteurs  Top 
  • Noppe, H., meer
  • Arijs, K., meer
  • De Wasch, K., meer
  • Van Cruchten, S.
  • Poelmans, S., meer
  • Courtheyn, D.
  • Cobbaert, E.
  • Gillis, W.
  • Vanthemsche, P.
  • De Brabander, H.F., meer
  • Janssen, C.R., meer
  • Van Hoof, N.

Abstract
    Continuous production of new products used as growth promotors in animal husbandry, sports doping and products for body-building, requires residue laboratories to initiate research on developing a strategy for the identification of ‘unknown’ components. In this study, a strategy for elucidating the identity, the structure and the possible effects of illegal estrogenic compounds in an unidentified water based solution is presented. To obtain complete information on the composition and activity of the unidentified product a multi-disciplinary approach was needed. A case-study is described with a ‘solution X’ found during a raid. At first, in vivo techniques (animal trials with mice, anatomical and histological research) were combined with in vitro techniques (the yeast estrogenic screen (YES)). In a later state of the investigation, HPLC fractionation, liquid chromatography-multiple mass spectrometry (LCMS- MS) and gas chromatography-multiple mass spectrometry (GC-MS-MS) were used. Finally, the identity of ‘solution X’ was confirmed in a low concentration range (10 ng.l-1 E1 and 400 ng.l-1 EE2).

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