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Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica)
Citatie
Vick-Majors T, Priscu J, Amaral-Zettler L (2019): Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica&v=1.2 https://dx.doi.org/10.15468/axqk7g

Toegang tot data
Gearchiveerde data
Beschikbaarheid: Creative Commons License Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.

Beschrijving
Amplicon sequencing dataset (454 pyrosequencing) of Archaea (16S), Bacteria (16S) and Eukaryote (18S) in the water column of lake Fryxell and Lake Bonney, in the McMurdo Dry valleys (Antarctica). meer

Duplicate samples were collected at depths of 6 m (bacterial and primary production maximum) and 9 m (chemocline; chlorophyll-a maximum) in lake Fryxell (2 November 2007 and 25 March 2008) and 13 m (chemocline; bacterial production, chlorophyll-a, primary production maximum) and 18 m (hypersaline, bottom of trophogenic zone) in lake Bonney (west Lobe; 30 November 2007 and 12 March 2008). Samples for DNA extraction were filtered onto 0.2-μM Sterivex filters (Millipore, Billerica, MA, USA) and stored with 2.0 ml of Puregene lysis buffer at −20 °C until further processing.
Study Extent: Sampling occurred during the Austral summer and autumn in lake Fryxell and lake Bonney, Antarctica
Method step description:
  1. DNA extraction protocols can be found at http://amarallab.mbl.edu.
  2. The V6 hypervariable regions of Archaa and bacteria were amplified, while for Eukaryota, amplification of the V9 hypervariable region followed established protocols (Amaral-Zettler et al., 2009). We multiplex-sequenced the resulting amplicons using bar-coded primers (Huber et al., 2007; Amaral-Zettler et al., 2009) on a 454 Genome Sequencer FLX (Roche, Switzerland) using the manufacturer’s recommended protocol.

Scope
Kernwoorden:
Zoet water, Dna sequencing, Metadata, Antarctica, Victoria Land, Bonney L., Antarctica, Victoria Land, Fryxell L., Antarctica, Victoria Land, McMurdo Dry Valleys, Archaea, Bacteria

Geografische spreiding
Antarctica, Victoria Land, Bonney L. [Marine Regions]
Antarctica, Victoria Land, Fryxell L. [Marine Regions]
Antarctica, Victoria Land, McMurdo Dry Valleys [Marine Regions]

Spreiding in de tijd
22 November 2007 - 25 Maart 2008

Taxonomische spreiding
Archaea [WoRMS]
Bacteria [WoRMS]

Parameter
Moleculaire data

Bijdrage door
Marine Biological Laboratory; The Josephine Bay Paul Center in Camparative Molecular Biology and Evolution, meerdata creator
Montana State University, meerdata creator

Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2019-04-04
Informatie laatst gewijzigd: 2019-04-10
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