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Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils
Citatie
Niederberger T, Sohm J, Gunderson T, Parker A, Tirindelli J, Capone D, Carpenter E, Cary S (2019): Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils&v=1.2 https://dx.doi.org/10.15468/y7hujy

Toegang tot data
Gearchiveerde data
Beschikbaarheid: Creative Commons License Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.

Beschrijving
Amplicon sequencing dataset of Bacteria (16S ssu rRNA gene) and Eukaryotes (18S ssu rRNA gene) from wet and dry soil samples near Miers stream, Nostoc pond, Miers lake and Buddha lake, in the McMurdo Dry Valleys, Antarctica. meer

Soil samples were collected aseptically at each site into sterile Whirl-Pak bags using a sterile metal spatula and kept frozen during transport to the laboratory.
Study Extent: Samples were collected from the Miers Valley region of Antarctica during the summer months, including: MS1, Miers Stream 1 (S78°05.748′, E163°44.822′), a site located at the edge of the runoff stream flowing from Miers Glacier into Miers Lake in Miers Valley; ML1, Miers Lake 1 (S78°05.615′, E163°49.912′) located on the north side of Miers Lake; BL, Baby Buddha Lake (S78°03.621′, E163°46.463′), and NP, Nostoc Pond (S78°03.920′, E163°46.497′) adjacent to lake systems located over the north ridge of Miers Valley.
Method step description:
  1. DNA was isolated from homogenized soil samples using the PowersoilTM DNA isolation kit (MOBIO) as per manufacturer’s instructions with all terminal-restriction fragment length polymorphisms (T-RFLPs) based on pooled triplicate PCR assays.
  2. Tagged amplicon pyrosequencing of the V1 to V3 region of the 16S and 18S rRNA gene (primer pairs 28F-519R and euk7F-euk570R, respectively) was performed on DNA extracts by the Research and Testing Laboratories located in Lubbock, TX (www.researchandtesting.com) using FLX technology (Roche).

Scope
Kernwoorden:
Dna sequencing, Metadata, Rrna, Antarctica, Victoria Land, McMurdo Dry Valleys, Bacteria

Geografische spreiding
Antarctica, Victoria Land, McMurdo Dry Valleys [Marine Regions]

Spreiding in de tijd
18 Januari 2009 - 8 December 2009

Taxonomische spreiding
Bacteria [WoRMS]

Parameter
Moleculaire data

Bijdrage door
San Francisco State University, meerdata creator
University of Delaware, meerdata creator
University of Southern California (USC), meerdata creator

Gerelateerde datasets
Gepubliceerd in:
AntOBIS: Antarctic Ocean Biogeographic Information System, meer

Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2019-04-03
Informatie laatst gewijzigd: 2019-04-10
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