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Bacteria (16S ssu rRNA) in an Antarctic snow sample
Citatie
Michaud L, Lo Giudice A, Mysara M, Monsieurs P, Raffa C, Leys N, Almafitano S, Van Houdt R (2019): Bacteria (16S ssu rRNA) in an Antarctic snow sample. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacteria_antarctic_snow&v=1.2 https://doi.org/10.15468/lp7r84
Contact:
Michaud, Luigi ;
Lo Giudice, Angelina Beschikbaarheid: Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.
Beschrijving
Amplicon sequencing sample of Bacteria (16S ssu rRNA gene, v1-v3 region) from a snow sample taken from the "clean Area”, 2 km South from the Antarctic Research Base “Concordia” (75°06′S–123°20′E). meer
Sampling was performed by using polyethylene boxes pre-treated with 1M hydrogen chloride and hydrogen peroxide. Sterile gloves and suit, and an ethanol flame-sterilized shovel were used. Study Extent: Snow surface sample was collected in triplicate from a “Clean Area” 2 km from the Research Base “Concordia” (75°06′S–123°20′E) Quality Control: Autoclave-sterilized Milli-Q water was treated in tandem with the snow samples as a negative-control field blank. Quantity and quality of extracted DNA was checked by nanodrop ND-1000 device and the Quant-iT PicoGreen dsDNA reagent and kit (Life Tech, Carlsbad, USA) following the manufacturer's instructions. Method step description:
Scope Kernwoorden: Terrestrisch, Dna sequencing, Metadata, Snow, Antarctica, Bacteria Geografische spreiding Antarctica [Marine Regions] Spreiding in de tijd
Vanaf 2010 [Afgelopen] Taxonomic coverage
Bacteria [WoRMS]
Parameter
Moleculaire data Bijdrage door
Gerelateerde datasets
Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2019-03-28
Informatie laatst gewijzigd: 2019-04-10
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